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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/04/2004 |
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Data da última atualização: |
16/04/2004 |
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Autoria: |
ALVAREZ, E.; CHACON, M. I.; SANCHEZ, J. |
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Título: |
DNA polymorphism and virulence variation of Phytophthora population isolated from cassava Manihot esculenta Crantz. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p. 37, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Cassava (Manihot esculenta Crantz) is the fourth most important source of dietary carbohydrate in developing countries. A major constraint to cassava production in Latin America is rot, a destructive and widespread disease. To effectively breed for root-rot resistance, researchers must understand the nature of root rotcausing pathogens in general and the virulence patterns of Phytophthora in particular. The overall objective of this research was to study the genetic variation of Phytophthora isolates in terms of virunlence and DNA polymorphism. One hundred twenty two Phytophthora isolates were obtained using a direct palting method and a baiting technique in which fragments of cassava plantlets propagated in vitro, were used as bait. All isolates were characterized by morphology, pathogenicity and PCR-RAPD analysis. Seven reference isolates of different Phytophthora species were also included in the molecular analysis(I). We also identified the isolates by enzyme-linked immunosorbent assay (ELISA) and DNA hybridization, using a Phytophthora specific probe(2). Virulence variation was determined by inoculating through wounding, cassava sprouts that represented 10 varietal differentials. The inocula were 30 selected isolates from different Colombian regions. Most isolates were not clearly differentiated on PDA, V8A and oatmeal agar by colony type or growth. Identification of the isolates by morphology was difficult because not all isolates produce fungal structures.Amplification of the ITS (internal Transcribed Spacer) region of the rDNA was obtained with template DNA from 122 isolates using extracted DNA. The amplified product for the ITS region of all species was 900 bp. Restriction digest with Aiul, Mspl and Hindl of the product amplified for the ITS region showed different restriction paftems, which corresponded to the different species tested. Five radom 10 mer primers (Operon Technologies, Inc.) gave reproducible bands. Polymorphisms with these single primers differentiated the isolates. Colombian Phytophthora populations are highly diverse and our isolates grouped into 12 clusters. All the isolates were detected by the DNA hybridization probe, a more sensitive method than the ELISA test for identifying the pathogens. The sysptoms caused by individuals were very similar, although sysmptom severity differed among isolates. Virulence was observed within the Colombian group of isolates, but no correlation between virulence variation and DNA polymorphism was observed. MenosAbstract: Cassava (Manihot esculenta Crantz) is the fourth most important source of dietary carbohydrate in developing countries. A major constraint to cassava production in Latin America is rot, a destructive and widespread disease. To effectively breed for root-rot resistance, researchers must understand the nature of root rotcausing pathogens in general and the virulence patterns of Phytophthora in particular. The overall objective of this research was to study the genetic variation of Phytophthora isolates in terms of virunlence and DNA polymorphism. One hundred twenty two Phytophthora isolates were obtained using a direct palting method and a baiting technique in which fragments of cassava plantlets propagated in vitro, were used as bait. All isolates were characterized by morphology, pathogenicity and PCR-RAPD analysis. Seven reference isolates of different Phytophthora species were also included in the molecular analysis(I). We also identified the isolates by enzyme-linked immunosorbent assay (ELISA) and DNA hybridization, using a Phytophthora specific probe(2). Virulence variation was determined by inoculating through wounding, cassava sprouts that represented 10 varietal differentials. The inocula were 30 selected isolates from different Colombian regions. Most isolates were not clearly differentiated on PDA, V8A and oatmeal agar by colony type or growth. Identification of the isolates by morphology was difficult because not all isolates produce fungal structures.Am... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 03001naa a2200145 a 4500
001 1651914
005 2004-04-16
008 1998 bl uuuu u00u1 u #d
100 1 $aALVAREZ, E.
245 $aDNA polymorphism and virulence variation of Phytophthora population isolated from cassava Manihot esculenta Crantz.
260 $c1998
520 $aAbstract: Cassava (Manihot esculenta Crantz) is the fourth most important source of dietary carbohydrate in developing countries. A major constraint to cassava production in Latin America is rot, a destructive and widespread disease. To effectively breed for root-rot resistance, researchers must understand the nature of root rotcausing pathogens in general and the virulence patterns of Phytophthora in particular. The overall objective of this research was to study the genetic variation of Phytophthora isolates in terms of virunlence and DNA polymorphism. One hundred twenty two Phytophthora isolates were obtained using a direct palting method and a baiting technique in which fragments of cassava plantlets propagated in vitro, were used as bait. All isolates were characterized by morphology, pathogenicity and PCR-RAPD analysis. Seven reference isolates of different Phytophthora species were also included in the molecular analysis(I). We also identified the isolates by enzyme-linked immunosorbent assay (ELISA) and DNA hybridization, using a Phytophthora specific probe(2). Virulence variation was determined by inoculating through wounding, cassava sprouts that represented 10 varietal differentials. The inocula were 30 selected isolates from different Colombian regions. Most isolates were not clearly differentiated on PDA, V8A and oatmeal agar by colony type or growth. Identification of the isolates by morphology was difficult because not all isolates produce fungal structures.Amplification of the ITS (internal Transcribed Spacer) region of the rDNA was obtained with template DNA from 122 isolates using extracted DNA. The amplified product for the ITS region of all species was 900 bp. Restriction digest with Aiul, Mspl and Hindl of the product amplified for the ITS region showed different restriction paftems, which corresponded to the different species tested. Five radom 10 mer primers (Operon Technologies, Inc.) gave reproducible bands. Polymorphisms with these single primers differentiated the isolates. Colombian Phytophthora populations are highly diverse and our isolates grouped into 12 clusters. All the isolates were detected by the DNA hybridization probe, a more sensitive method than the ELISA test for identifying the pathogens. The sysptoms caused by individuals were very similar, although sysmptom severity differed among isolates. Virulence was observed within the Colombian group of isolates, but no correlation between virulence variation and DNA polymorphism was observed.
700 1 $aCHACON, M. I.
700 1 $aSANCHEZ, J.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv. 17, p. 37, nov., 1998. Suplemento.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
24/10/2000 |
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Data da última atualização: |
31/03/2017 |
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Autoria: |
POTT, V. J.; POTT, A.; DANTAS, M. |
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Afiliação: |
EMBRAPA Pantanal (Corumba, MS). |
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Título: |
Dinamica da vegetacao aquatica de vazante, fazenda Campo Dora, Nhecolandia, Pantanal, MS. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE BOTANICA, 51., 2000, Brasilia. Resumos... Brasilia: Sociedade Botanica do Brasil, 2000. p.233. |
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Idioma: |
Português |
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Conteúdo: |
Na Nhecolandia, segunda maior sub-regiao do Pantanal, ha dois padroes de ambientes aquaticos: lagoas e vazantes, respectivamente de drenagem fechada e aberta. As vazantes sao linhas de aporte e drenagem de aguas de chuva nos campos, a montante, em conexao com rios durante a cheia. Em geral, ha partes espraiadas, com vegetacao de gramineas na seca e aquaticas na cheia, e partes canalizadas, com mata de galeria de Licania parviflora Hub. Sobre a vegetacao de vazantes ha pouca informacao. Este trabalho tem o objetivo de descrever a vegetacao aquatica de vazante na epoca de cheia (fevereiro 1999), e de seca (setembro 1999). Estabeleceu-se uma transeccao perpendicular, fixa, em 4 areas, partindo da borda ao centro das vazantes. Com fita metrica sobre a vegetacao aquatica, em quadrados de 1 m2, distribuidos ao longo da transeccao, anotaram-se a profundidade e a cobertura das especies. As principais especies em frequencia, na cheia, foram: Eleocharis minima Kunth (70%), Leersia hexandra Sw. e Panicum laxum Sw. (45%), Luziola subintegra Swallen (42%), Bacopa myriophylloides (Benth.) Wettst. e Utricularia gibba L. (37,5%). Na seca foram: Eichhornia azurea (Sw.) Kuntz (67,5%), uziola subintegra (62,5%), Utricularia gibba (52,5%), Eleocharis minima (37,5%) e Pontederia parviflora Aleander (30%). A grande frequencia de E. azurea na seca, deve-se ao fato desta ficar concentrada nas depressoes maiores das vazantes que nao secaram completamente. As prfoundidades minimas na seca variaram entre 18 a 93 cm, e na cheia 62 a 136 cm. Comparando com trabalhos feitos em lagoas proximas a area estudada, detectaram-se especies que nao ocorrem ali, como: Nymphaeae oxypetala Planch., Bacopa reflexa (Benth.) Edwall e Paspalum morichalense Davidse, Zul., Filg., especies de ambientes loticos. MenosNa Nhecolandia, segunda maior sub-regiao do Pantanal, ha dois padroes de ambientes aquaticos: lagoas e vazantes, respectivamente de drenagem fechada e aberta. As vazantes sao linhas de aporte e drenagem de aguas de chuva nos campos, a montante, em conexao com rios durante a cheia. Em geral, ha partes espraiadas, com vegetacao de gramineas na seca e aquaticas na cheia, e partes canalizadas, com mata de galeria de Licania parviflora Hub. Sobre a vegetacao de vazantes ha pouca informacao. Este trabalho tem o objetivo de descrever a vegetacao aquatica de vazante na epoca de cheia (fevereiro 1999), e de seca (setembro 1999). Estabeleceu-se uma transeccao perpendicular, fixa, em 4 areas, partindo da borda ao centro das vazantes. Com fita metrica sobre a vegetacao aquatica, em quadrados de 1 m2, distribuidos ao longo da transeccao, anotaram-se a profundidade e a cobertura das especies. As principais especies em frequencia, na cheia, foram: Eleocharis minima Kunth (70%), Leersia hexandra Sw. e Panicum laxum Sw. (45%), Luziola subintegra Swallen (42%), Bacopa myriophylloides (Benth.) Wettst. e Utricularia gibba L. (37,5%). Na seca foram: Eichhornia azurea (Sw.) Kuntz (67,5%), uziola subintegra (62,5%), Utricularia gibba (52,5%), Eleocharis minima (37,5%) e Pontederia parviflora Aleander (30%). A grande frequencia de E. azurea na seca, deve-se ao fato desta ficar concentrada nas depressoes maiores das vazantes que nao secaram completamente. As prfoundidades minimas na seca variaram en... Mostrar Tudo |
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Palavras-Chave: |
Aquatic vegetation; Dinamic; Dinamica; Vazante; Vegetacao aquatica. |
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Thesaurus NAL: |
Pantanal. |
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Categoria do assunto: |
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Marc: |
LEADER 02444naa a2200217 a 4500
001 1803673
005 2017-03-31
008 2000 bl --- 0-- u #d
100 1 $aPOTT, V. J.
245 $aDinamica da vegetacao aquatica de vazante, fazenda Campo Dora, Nhecolandia, Pantanal, MS.
260 $c2000
520 $aNa Nhecolandia, segunda maior sub-regiao do Pantanal, ha dois padroes de ambientes aquaticos: lagoas e vazantes, respectivamente de drenagem fechada e aberta. As vazantes sao linhas de aporte e drenagem de aguas de chuva nos campos, a montante, em conexao com rios durante a cheia. Em geral, ha partes espraiadas, com vegetacao de gramineas na seca e aquaticas na cheia, e partes canalizadas, com mata de galeria de Licania parviflora Hub. Sobre a vegetacao de vazantes ha pouca informacao. Este trabalho tem o objetivo de descrever a vegetacao aquatica de vazante na epoca de cheia (fevereiro 1999), e de seca (setembro 1999). Estabeleceu-se uma transeccao perpendicular, fixa, em 4 areas, partindo da borda ao centro das vazantes. Com fita metrica sobre a vegetacao aquatica, em quadrados de 1 m2, distribuidos ao longo da transeccao, anotaram-se a profundidade e a cobertura das especies. As principais especies em frequencia, na cheia, foram: Eleocharis minima Kunth (70%), Leersia hexandra Sw. e Panicum laxum Sw. (45%), Luziola subintegra Swallen (42%), Bacopa myriophylloides (Benth.) Wettst. e Utricularia gibba L. (37,5%). Na seca foram: Eichhornia azurea (Sw.) Kuntz (67,5%), uziola subintegra (62,5%), Utricularia gibba (52,5%), Eleocharis minima (37,5%) e Pontederia parviflora Aleander (30%). A grande frequencia de E. azurea na seca, deve-se ao fato desta ficar concentrada nas depressoes maiores das vazantes que nao secaram completamente. As prfoundidades minimas na seca variaram entre 18 a 93 cm, e na cheia 62 a 136 cm. Comparando com trabalhos feitos em lagoas proximas a area estudada, detectaram-se especies que nao ocorrem ali, como: Nymphaeae oxypetala Planch., Bacopa reflexa (Benth.) Edwall e Paspalum morichalense Davidse, Zul., Filg., especies de ambientes loticos.
650 $aPantanal
653 $aAquatic vegetation
653 $aDinamic
653 $aDinamica
653 $aVazante
653 $aVegetacao aquatica
700 1 $aPOTT, A.
700 1 $aDANTAS, M.
773 $tIn: CONGRESSO NACIONAL DE BOTANICA, 51., 2000, Brasilia. Resumos... Brasilia: Sociedade Botanica do Brasil, 2000. p.233.
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